Figure 2.

GC1 and GCAP1 expression and localization in the photoreceptors of untreated and treated 4Bnr rd3 mice by immunofluorescence microscopy. The right eye of a 4Bnr rd3 mouse was injected (treated) with AAV8(Y733F)-hGRK1-mRd3 at P14 with the uninjected (untreated) left eye serving as a contralateral control. Retinal cryosections prepared 2 weeks post-injection (P28) were labeled for GC1 or GCAP1 (green) and counterstained with DAPI (blue) nuclear stain. (A) Retinal sections from a whole eye at low magnification. GC1 staining is present throughout the retina of the treated, but not the untreated eye. Bar 200 μm. (B) GC-labeled retinal sections at higher magnification. GC1 is correctly localized to the rod and cone (arrow) OSs of the treated eye similar to GC1 localization in WT Balb/c mouse retina. (C) GCAP1-labeled retinal sections at higher magnification. GCAP1 distribution in the treated retina is similar to that in the WT retina, whereas the untreated retina shows reduced GCAP1 expression primarily in the inner segment. Representative micrographs are shown from analysis of six mice. OS, OS layer; IS, inner segment layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. Bar—10 μm.