Figure 5.

ERGs of AAV8(Y733F)-hGRK1-mRd3-treated and -untreated 4Bnr rd3 mice. Mice were treated at P14 and their dark-adapted (scotopic) and light-adapted (photopic) ERGs were measured at various times after treatment. (A) Typical scotopic ERG ellicited at a light intensity of 2.25 cd s/m² and photopic ERG ellicited at a light intensity of 12 cd.s/m² in the presence of background light of 30 cd/m². Scotopic response was observed for the untreated and treated eyes at 7 days post-injection. At 3 months post-injection an ERG response was only observed for the treated eye. Photopic response was not observed at any age for the untreated or treated eyes. ERGs of WT are shown as a reference. (B) Effect of a-wave and b-wave amplitudes (mV) of the scotopic response as a function of the age of the WT, treated and untreated mice. Data are expressed as mean ± SD for n ≥ 3.