Figure 6.

Presence of cone photoreceptors in the retina of AAV8(Y733F)-hGRK1-mRd3-treated and -untreated mice as visualized by immunofluorescence microscopy. Retinal cryosections from WT mice and the treated and untreated 4Bnr mice were were double labeled for GC1 and middle wavelength cone opsin. Both the WT and treated mice showed a significant number of cone photoreceptor cells with cone opsin and GC1 co-localized to the OSs (merged image). The treated mice were injected at P14  and analyzed 115 days later. No significant labeling was observed for the untreated 4Bnr mice indicative of extensive cone degeneration. Representative micrographs are shown from the analysis of six 4Bnr mice and three WT mice analyzed.