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. 2013 Aug 29;5:15. doi: 10.1186/2045-824X-5-15

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Copyright © 2013 Walton et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemical staining of Tet-regulated transgene expressing murine hearts. Eight week old mice were left uniduced or induced to express the stable HIF-1α-PPN-HA transgene over four weeks. A. HA immunohistological staining while left uninduced (On dox), B. HA immunological staining following doxycycline removal and transgene induction for; three days (3 days off dox), C. seven days (7 days off dox), and D. twenty-eight days (28 days off dox). A clear and progressive build-up of the stable HIF-1α-PPN-HA transgene can be seen over the course of four weeks. All images (20X); HA=hemagglutinin.