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. 2013 Sep 3;9:172. doi: 10.1186/1746-6148-9-172

Table 4.

Intra-assay performance of the TaqMan probe based real time PCR assays

 
 Reaction 1
 Reaction 2
 Reaction 3
Theoretical copy number Average Ct Average calculated copy number CV % Average Ct Average calculated copy number CV % Average Ct Average calculated copy number CV %
MVV assay
10 6
 20
 853389
 8.67%
 20.04
 1008108
 23.29%
 19.83
 1007657
 7.96%
10 5
 23.29
 105939
 9.25%
 23.40
 102693
 9.62%
 23.32
 97177
 9.14%
10 4
 26.69
 12190
 3.14%
 26.66
 11365
 1.43%
 26.64
 10483
 7.21%
10 3
 30.29
 1248
 6.60%
 30.07
 1136
 5.75%
 30.16
 990
 7.14%
10 2
 34.65
 83
 37.84%
 34.01
 79
 1.35%
 33.59
 103
 34.05%
CAEV assay
10 5
 21.22
 87689
 38.76
 20.96
 94514
 31.38
 21.71
 92044
 30.09
10 4
 23.55
 17265
 18.81
 24.41
 11326
 17.53
 24.91
 11230
 16.01
10 3
 27.33
 1463
 13.65
 28.16
 1160
 20.64
 27.94
 1193
 3.18
10 2
 31.15
 66
 39.76
 32.58
 79
 17.85
 33.06
 55
 1.84
10 34.4 11 46.69 35.73 13 58.26 35.56 11 2.3