Table II.

Significant temperatures for the plus-format isoforms

Isoform	AI-1	AI-2	GI	DI	ZI	EI	OI
TcRI (extrapolation) °C ± SDa	24.3 ± 1.1	34.0 ± 0.67	44.8 ± 0.72	52.8 ± 0.34	57.7 ± 0.6	64.2 ± 0.52	71.0 ± 0.47
Conversion Tc °Cb	–	–	45.0	53.0	58.0	64.5	70.0
TcOD °Cc	23.0	34.0	45.0	52.5	57.0	65.0	72.0
Assay T°C (OD700) to distinguish from next lowerd	20.0	28.0	41.0	49.0	55.0	60.0	68.0
Convenient annealing T°C	∼20	∼20	∼37	∼37	∼37	∼50	∼50

^aExtrapolation temperatures (RI: Figures 4 and 5).

^bOptimum conversion temperature to obtain the indicated isoform (Figure 2).

^c50% OD₇₀₀ thermal transition points (Figure 6).

^dOD₇₀₀ assay temperatures.