Figure 3.

Enhanced RA signaling in T cells in tumor-bearing mice. A, comparative RA signaling in CD8⁺ T cells, CD4⁺ T cells, and CD11c⁺ DCs in tumor-bearing and naïve mice. On day 6 posttumor inoculation, CD8⁺ T cells, CD4⁺ T cells, and CD11⁺ DCs were isolated from tumor tissue (TIL) of B16.OVA-bearing DR5-Luciferase (Luc⁺) or littermate control (Luc⁻), TDLN and spleen of B16.OVA-bearing or naïve DR5-Luciferase mice (pooled ≥7 mice per group), and total cellular luciferase activity was measured using 5 × 10⁵ cells per well. Bar graphs for TILs show single well without error bars. About LN and spleen, bar graphs show triplicate wells with mean ± SD. Data shown are representative of 2 experiments with similar results. B, heightened RA reporting of tumor specific T cells in the TME of tumor-bearing mice. Representative imaging of tumor site of day 6 B16.OVA-bearing C57B L/6 mice receiving adoptive transfer of CD8⁺ T cells from OTI^Luc mice. C, quantified RA reporting of CD8⁺ TIL. WBI was used to quantify the total photon flux of infiltrating OTI^Luc T cells of the RA reporting signal at the tumor site as shown in (B). Shown is pooled data from 2 experiments with total n ≥ 11 mice per group. In A and C, statistically significant differences were determined by t test.