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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Cell Host Microbe. 2013 May 15;13(5):558–569. doi: 10.1016/j.chom.2013.03.011

Figure 5. The IRE1-RIDD Pathway Defines a General Rule for Innate Immune Surveillance by the ER.

Figure 5

(A) Wild-type MEFs or MEFs lacking IRE1α (IRE1α−/−) or RIG-I (RIG-I−/−) were intoxicated with an enzymatically inactive Shiga toxin mutant (StxE) or its B subunit alone (STxB), and IL-6 mRNA was quantified.

(B and C) T84 or Caco-2 cells pretreated or not with 4µ8C were infected with SV40 virus (B) or Anthrax-fused Diphtheria (C) for 4h and immune responses were assessed by qPCR.

(D) Wild-type macrophages or MAVS knockout macrophages pretreated or not with 4µ8C were intoxicated with toxins for 4 hr and analyzed for IL-6 by qPCR.

Data are shown as means ± SEM. PA, protective antigen; LFN, amino-terminal 255 residues of lethal factor; LFNDTA*, fusion protein composed of LFN and the inactive catalytic domain of diphtheria toxin; LFNDTA, fusion protein composed of LFN and the catalytic domain of diphtheria toxin. Other nomenclature is as in Figures 1 and 4.