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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: J Control Release. 2013 May 3;171(3):349–357. doi: 10.1016/j.jconrel.2013.04.018

Figure 3.

Figure 3

Complexation of siRNA with NLC. (A) A representative agarose gel electrophoresis image illustrating siRNA complexation efficiency by LHRH-NLC-TAX at the following w/w ratio (weight NLC/weight siRNA): (1) 0:1 (naked siRNA, control); (2) 10:1; (3) 50:1; (4) 100:1; and (5) 117:1. Complexation of siRNA prevented staining of siRNA by ethidium bromide and led to the disappearance of the siRNA band. Therefore, the fluorescent intensity of the siRNA band on the gel (well 5) disappeared when siRNA was complexed with NLC at w/w ratio = 117:1. (B) siRNA complexation efficiency of LHRH-NLC-TAX evaluated by the ethidium bromide dye displacement assay. The arrow on the curve highlights the w/w ratio corresponding to the apparent end of siRNA complexation with NLC. Means ± SD are shown.