Skip to main content
. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 18;0:249–256. doi: 10.1016/j.freeradbiomed.2013.04.019

Fig. 3.

Fig. 3

Effects of vitamin C, AG and catalase on the activity of PARP-1 in arsenite treated cells. HaCat cells were treated with arsenite as described in “Methods” except the indicated concentration of either vitamin C or AG was added for 30 min before arsenite addition. In catalase experiment, HEKn cells were treated with indicated concentrations of catalase for 30 min before arsenite addition. After incubation for 48 hours, PARP-1 was isolated by immunoprecipitation and the activity of PARP-1 was measured with a HT Colorimetric PARP/Apoptosis Assay kit. A) Effects of vitamin C on the activity of PARP-1. B) Effects of AG on the activity of PARP-1. C) Effects of catalase on the activity of PARP-1. Data are presented as means ± S.D. * P<0.05 vs. As alone group, n=6.