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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 18;0:249–256. doi: 10.1016/j.freeradbiomed.2013.04.019

Fig. 4.

Fig. 4

Effects of arsenite, hydrogen peroxide, or NONOate on PARP-1 DNA binding. Cells were treated with indicated concentrations of sodium arsenite, hydrogen peroxide, or NONOate for 48 hrs. PARP-1 was isolated by immunoprecipitation and PARP-1 binding to DNA was assayed by EMSA. A) Images of EMSA results after electrophoresis, visualization of nucleic acids with SYBR green. B) Effects of sodium arsenite on the DNA-binding of PARP-1. C) Effects of hydrogen peroxide on the DNA-binding of PARP-1. D) Effects of NONOate on the DNA-binding of PARP-1. E) Effects of vitamin C (400 μM) and AG (400 μM) on the DNA-binding of PARP-1 with arsenite (2 μM). Data are presented as means± S.D. * P<0.05 vs. concentration at 0 group, n=4.