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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 18;0:249–256. doi: 10.1016/j.freeradbiomed.2013.04.019

Fig. 5.

Fig. 5

Effects of arsenite, hydrogen peroxide, NONOate on the zinc content of PARP-1 isolated from treated HaCat cells. Cells were treated with sodium arsenite, hydrogen peroxide, or NONOate for 48 hrs with or without pretreatment with vitamin C (400 μM) or AG (400 μM). PARP-1 was isolated from the treated cells by immunoprecipitation. The content of zinc in the isolated PARP-1 was detected by spectrophotometric method. Data were presented as means ± S.D., * P<0.05 vs. 0 group, n=6. A) Effects of arsenite; B) Effects of hydrogen peroxide; C) Effects of NONOate; D) Effects of arsenite (2 μM) with or without pretreatment with vitamin C (400 μM) or AG (400 μM).