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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 18;0:249–256. doi: 10.1016/j.freeradbiomed.2013.04.019

Fig. 6.

Fig. 6

Effects of arsenite, hydrogen peroxide, or NONOate on the activity of PARP-1 isolated from untreated HaCat cells. HaCat cell lysates were prepared from untreated, subconfluent cells then PARP-1 was isolated by immunoprecipitation. The isolated PARP-1 was treated with the indicated concentrations of sodium arsenite, hydrogen peroxide, or NONOate at 4°C for 48 hrs before the activity of PARP-1 was determined using an HT Colorimetric PARP/Apoptosis Assay kit. Data were presented as means ± S.D., * P<0.05 vs. 0 group, n=6. A) Effects of arsenite. B) Effects of hydrogen peroxide. C) Effects of NONOate.