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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 18;0:249–256. doi: 10.1016/j.freeradbiomed.2013.04.019

Fig 8.

Fig 8

Effects of arsenite, hydrogen peroxide, or NONOate on the zinc content of purified PARP-1. PARP-1 protein was isolated from untreated HaCat cells by immunoprecipitation, then the isolated PARP-1 was treated with sodium arsenite (50 μM), hydrogen peroxide (50 μM), and NONOate (20 μM) for 48 hrs at 4°C The zinc content in the isolated PARP-1 was detected spectrophotometrically (DU 800, Beckman Coulter). Data were presented as means ± S.D., * P<0.05 vs. control group; n=6.