Table 2.
Summary of FGF23 protein expression by immunohistochemistry 1 of 2 plastic-embedded femur sections from FA- and vehicle-injected animals.
| Femur area examined |
Treatment | p value | |
|---|---|---|---|
| Folic acid | Vehicle | ||
| Cortex | |||
| Average score |
2.25±0.41 | 1.125±0.30 | <0.05 |
| Positive samples |
7/8 | 6/8 | |
|
| |||
| Trabeculae | |||
| Average score |
1.25±0.67 | 0.5±0.19 | 0.30 |
| Positive samples |
3/8 | 4/8 | |
Cortical staining was scored on a scale of 0 to 3+. Trabecular staining was scored by counting the number of osteocytes positive for FGF23 in the whole section. Two animals had neither cortical nor trabecular staining (both vehicle-injected); all others had either cortical or trabecular staining or both detectable (i.e. at least a 1+ score in cortex or 1 positive osteocyte in the trabecular area). Average staining shown are mean±SEM. P value is for vehicle-injected versus FA-injected animals. (N=8 animals for each group).