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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Neurochem. 2013 Jun 10;126(6):715–726. doi: 10.1111/jnc.12315

Fig. 4. Identification of the photolabeled residues in Munc13-1 C1 by LC/MS/MS after tryptic digest.

Fig. 4

A) Glu-582 as the site for 3-azibutanol: MS/MS data for the 3-azibutanol (0.5 mM) modified 16-mer peptide TATTPTYCYECEGLLW. In addition to the singly charged ions, a doubly charged b8++ (m/z 549) ion is also observed. B) Identification of Glu-582 as the site for 3-azioctanol: MS/MS data for the 3-azioctanol (3 mM) modified heptamer peptide CYECEGLLW. In addition to the singly charged ions, two doubly charged b7++ (m/z 521) and b8++ (m/z 577) ions are also observed. At the top of the each figure, the predicted charge/mass ratio of N-terminal ions (b ions) and C-terminal ions (y ions) are shown above and below the sequence, respectively. They are singly charged unless noted otherwise. The horizontal arrows show which m/z values for the b ions (above) and y ions (below) have a mass of 72 Da for 3-azibutanol and 128 Da for 3-azioctanol added to them. Observed values are shown in bold.