Table 2.
Cytotoxicity of D2C7 immunotoxin toward various cancer cells*
| Cell line | Cancer type | D2C7-(scdsFv)-PE38KDEL IC50 ng/ml |
|---|---|---|
| A431 (EGFRwt) | Epidermoid carcinoma | 0.180 ± 0.014 |
| 43 (EGFRwt) | Glioblastoma | 2.28 ± 0.85 |
| D08-0493MG (EGFRwt) | Glioblastoma | 2.5 ± 0.99 |
| D2159MG (EGFRwt and EGFRvIII) | Glioblastoma | 0.204 ± 0.220 |
| D270MG (EGFRwt and EGFRvIII) | Glioblastoma | 0.265 ± 0.134 |
*
All the cells are of human origin. Cytotoxicity data are given as an IC50 value; the concentration of immunotoxin that causes a 50% inhibition of protein synthesis after a 20-h incubation with immunotoxin. All the assays were performed in triplicate for each cell line. Results are presented as mean ± standard deviation where n ≥ 2.