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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Trends Cardiovasc Med. 2013 May 10;23(8):294–300. doi: 10.1016/j.tcm.2013.04.003

Fig. 2. Fate mapping of endocardial cells during EMT and early valve elongation.

Fig. 2

A. A schematic of fate mapping of cushion endocardial cells expressing a high level of Nfatc1 (Nfatc1h) using the Nfatc1-enhancer Cre (Nfatc1enCre) and Rosa26-lacZ Cre reporter (R26fslz) mice.

B. X-gal staining of E10.5Nfatc1enCre;R26fslz heart sections showing that Nfatc1h endocardial cell lineages (arrows) contribute to the cushion endocardium but not mesenchyme during EMT, indicating that Nfatc1h cells do not undergo EMT.

C and D. Showing that Nfatc1h endocardial cells contribute to the leading edge of the growing valve cups during elongation at E12.5. AV, atrioventricular; OFT, outflow tract. (Adapted from Wu et al. Circ. Res. 109;183-192, 2011).