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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Res. 2013 Jul 1;73(17):5371–5380. doi: 10.1158/0008-5472.CAN-12-4707

Figure 2.

Figure 2

Jade-1 regulates levels of endogenous phospho-AKT. A. Jade-1 silencing increased the level of endogenous phospho-AKT in HK-2 cells. This effect was blocked by wortmannin or LY294002. By immunoblotting, pErk1/2 and Erk1/2 were also not affected by Jade-1 silencing in the absence or presence of LY294002. B. The effect of Jade-1 silencing on phospho-AKT was suppressed by a silencing-resistant Jade-1 vector (pJade-1). C. Nonsi and Jade-1-sh1 cells were starved for 16 hours then stimulated with 20 ng/mL IGF-1 for times shown. D. Stable overexpression of Jade-1 in 786-O renal cancer cell clonal lines inhibited endogenous phospho-AKT. E. Stable expression of pVHL in renal cancer cells increases endogenous Jade-1 and decreases endogenous phospho-AKT. The 786-O stable cell line pools expressing either HA empty vector or HA-pVHL were examined for endogenous phospho-AKT four days, two days, and one day after confluence. F. The effect of pVHL on phospho-AKT is Jade-1 dependent. The 786-O VHL cells were transduced with nonsi, pJade-1sh1 or pJade-1sh2 containing virus and then examined for endogenous phospho-AKT.