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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Res. 2013 Jul 1;73(17):5371–5380. doi: 10.1158/0008-5472.CAN-12-4707

Figure 5.

Figure 5

AKT activity and levels of endogenous phospho-substrates of AKT are increased in Jade-1-silenced HK-2 cells. A. Endogenous AKT was immunoprecipitated from cellular extracts of HK-2 stable cell lines and then incubated with purified GSK3β substrate. The amount of phosphorylated GSK3β S9 was assessed by immunoblotting. B. Cell lysates from HK-2 cell lines were probed with a phospho-AKT substrate antibody. C. Levels of endogenous phospho-GSK3β S9, a known substrate of phospho-AKT, were assessed by immunoblot. D. Levels of endogenous p21 in the HK-2 cell lines were assessed without or with LY294002.