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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Pediatr Res. 2013 Jun 19;74(3):259–265. doi: 10.1038/pr.2013.103

Fig. 4.

Fig. 4

RA and RA+TNFα strengthen the expression of Math1 in cultured mMEEC. Incubation of cells with RA for 12~24 hours increased the expression of the Math1 mRNA transcripts by RT-PCR (A). FACS verified that RA, TNFα, and RA+TNFα for 12 hours increased Math1-positive cell numbers (B, n=2). Immunohistochemistry further confirmed that an increase of the Math1-positive cells and translocation into the nuclei after treatment with RA and TNFα in a time-dependent manner (within 2~12 hours, C). It is noted that both RA and TNFα had no effects on the Math1 translocation to the nuclei at 24 hour. EGF=20ng/mL, TNFα=20ng/mL, RA=1µM. Ctrl, control; bar=5 µm.