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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Neurochem. 2013 Jun 12;126(6):705–714. doi: 10.1111/jnc.12312

Figure 1. Immunohistochemical labeling.

Figure 1

Merged view of a fluorescence photomicrograph for both bNOS and leptin receptor immunohistochemical labeling of a section through the PPN of the rat. bNOS labeled cholinergic PPN cells as solid red cytoplasm (rhodamine filter). Leptin receptor labeling was evident as punctiform green label (FITC filter). This suggests that all or most cholinergic cells in the PPN bear leptin receptors, and that many non-cholinergic cells also bear leptin receptors. Calibration bar: 100 μm.