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. 2013 Aug 22;2013:838491. doi: 10.1155/2013/838491

Table 1.

Schematic summary of the best characterized mAbs against arboviral pathogens.

mAb Origin Reactivity Target protein Epitope Cloning strategy Format/isotype Neutralizing activity Reference
4.8A Human DENV1–4 E DII EBV transformation IgG IC50 (μg/mL): 
DENV1: 1.5
DENV2: >40
DENV3: 2.4
DENV4: >40		 [37]
D11C IC50 (μg/mL):  
DENV1: 1.5
DENV2: 1.0
DENV3: 10.2
DENV4: 1.6
1.6D IC50 (μg/mL):  
DENV1: 1.5
DENV2: 0.2
DENV3: 0.5
DENV4: 2.7
2H12 Murine DENV1–4 E DIII (AB loop: aa 314–317) Hybridoma from BALB/c mouse immunized with DENV2 E/DIII IgG2b IC50 nM: 
DENV1: 0.56–54
DENV3: 29
DENV4: 145		 [38]
C9 Murine/chimeric DENV2 E DIII Phage display of a chimeric murine hybridoma library VH1/Vκ1 PRNT50 
850 μg/mL		 [39]
4E11 Murine DENV1–4 E DIII (strand A: 308, 312 and strand G: 387, 389, 391) Hybridoma IgG2a/κ IC50 (μg/mL):  
DENV10.16
DENV2: 0.13
DENV-3: 8
DENV-4: 15		 [40]
4G2 Murine DENV1–4 E DII (fusion loop) Hybridoma from DENV2 E immunized mice IgG PRNT50 (μg/mL):  
DENV2: 15		 [39]
9F12 Murine DENV1–4, WNV E DIII (aa 305, 307, 310, 330; A strand and BC loop) Hybridoma from BALB/c mouse immunized with DENV-2 E/DIII IgG1k DENV1–4PRNT50: 
2 · 10−8–2 · 10−7 M		 [41]
2A10G6 Murine DENV1–4, YFV, WNV, JEV, TBEV E DII (Fusion loop: aa 98–101) Hybridoma from BALB/c mouse immunized with inactivated DENV2 IgG1 PRNT50 (μg/mL):  
DENV1: 2
DENV2: 1.5
DENV3: 2.1
DENV4: 1.8
YNF: 3.6
WNV: 46		 [36]
mAb11 Human DENV1–4, WNV, SLEV, YFV, JEV MVEV E DII (fusion loop) Phage display of human scFv Fusion protein scFv-Fc PRNT80 (μg/mL):  
WNV: 1.25
DENV2: 6.25		 [42, 43]
E16
(MGAWN1)		 Murine WNV E DIII (LR; aa 302–309) Hybridoma from immunized mice with WNV E IgG2b/humanized PRNT50: 4–18 ng
PRNT90: 53–297 ng		 [44, 45]
1A1D-2 Murine DENV1–3 E DIII (A strand: aa 307, 310 and 312) Hybridoma from immunized mice with different pH-treated virus IgG2a DENV2 PRNT50: 
2.1 nM		 [46, 47]
1F4 Human DENV1 E (virion) DI-DII Electrofusion of infected memory B cells from DENV-immune subjects with EBV IgG DENV1 IC50
0.11 μg/mL		 [48]
2D22 DENV2 DIII DENV2 IC50
0.08 μg/mL
5J7 DENV3 DI-DII DENV3 IC50
0.10 μg/mL
E105 Murine DENV1 E DIII (BC loop: G328, T329 and D330; DE loop: K361E and E362K; FG loop: K385)
 Hybridomas derived from C57BL/6 IFN-αβR−/− mice infected with DENV1 IgG PRNT50 DENV-1: 0.5–59.2 ng/mL [32]
E106 DIII (BC loop: G328, T329 and D330; DE loop: K361E and E362K; FG loop: K385; A strand: S305, K307, E309, K310, and E311)
 PRNT50 DENV-1: 0.6–59.2 ng/mL
E111 DIII (CC′ loop) PRNT50:  
3.8–25 μg/mL		 [49]
CR4374 Human WNV E DIII Phage display of scFv IgG library VH2-05/VL1e PRNT50: 
0.18 μg/mL
PRNT90:  
0.95 μg/mL		 [50]
CR4353 VH3-30/Vk3-A27 PRNT50: 
0.026 μg/mL
PRNT90 
36.4 μg/mL
1A5 Chimpanzee DENV1–4, WNV, JEV, LGTV E DII (aa G106) Phage display of Fab library from DENV1–4 infected chimpanzees Humanized IgG1 PRNT50 (μg/mL):  
DENV1: 0.48
DENV2: 0.95
DENV3: 3.2
DENV4: 4.3
WNV/DENV4: 3.8
JEV: 21
LGTV: 28		 [51]
mAb11 Human DENV1–4, WNV E DII (fusion loop, W101, G104, G106) scFv library scFv-Fc PRNT80 (μg/mL):  
DENV2: 6.25
WNV: 1.25		 [42, 43]
3B4C-4 Murine SLEV E 1a Hybridoma IgG SLEV PRNT: <1.7 [52, 53]
1B2C-5 SLEV 1b SLEV PRNT: <1.7
6B5A-2 SLEV 1c SLEV PRNT: 4.8
4A4C-4 SLEV 1d SLEV PRNT: 2.9
1B5D-1 SLEV, JEV 2 SLEV PRNT: <1.7
2B5B-3 SLEV, JEV, MVEV, WNV, YFV 3 SLEV PRNT: 2.3
2B6B-2 All Flavivirus 4a SLEV PRNT: <1.7
6B6C-1 All Flavivirus 4b (in DII) SLEV PRNT: 2.3
A3 Chimpanzee JEV E2 DI aa. K179 Phage display Humanized PRNT50
0.04–0.2 nM		 [54]
B2 DII aa. I126 Phage display PRNT50
0.02–2 nM
E3 DIII aa. G132 Phage display PRNT50
0.14–0.93 nM
FabTJE12B02 Human JEV E N/A Phage display Fab FRNT50 
50.2 µg/mL		 [55]
5F10 Human CHIKV E2 Domain B EBV transformation IgG1λ2 IC50 < 100 ng/mL [56]
8B10 Human CHIKV E1-E2 E2 Domain A EBV transformation IgG1k IC50 < 100 ng/mL
CHK-152 Murine CHIKV E2 aa 59 Hybridoma IgG2c IC50 1–3 ng/mL [57]
11E7 Murine CCHFV Gn C-ter Hybridoma IgG PRNT80 diluted 1/2560 [58]
4-39-CC Murine RVFV G2 Domain IV Hybridoma IgG PRNT80 diluted 1/20480–1/81920 [59]
2C9 Murine YFV E   Hybridoma IgG2a PRNT90: 
1/104 17D
1/105.2 Asibi		 [60]
5A
7A
R3(27)		 Human YFV E DI-DII Phage display ScFv PRNT90:
0.1–0.3 μg/mL		 [61]
13D6 Murine TBEV E DIII Hybridoma IgG/chimeric PRNT50: 1.9 μg/mL
FRNT50: 4.5 μg/mL
IC50
1.9–16.7 μg/mL		 [62]
3B4C-4
(Hy4-26C)		 Murine VEEV E2 aa 182–209 Hybridoma IgG/humanized PRNT70 (μg/mL):  
39.4–125		 [63]