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. Author manuscript; available in PMC: 2013 Sep 8.
Published in final edited form as: Toxicol Appl Pharmacol. 2008 Feb 5;229(2):206–214. doi: 10.1016/j.taap.2008.01.022

Fig. 3.

Fig. 3

Comparison of proteasome-inhibitory effects between DSF–Cd and MG132. A, Inhibitory effects on purified 20S proteasome by DSF–Cd and MG132 were tested. B, Intact PC-3 cells containing 26S proteasome were incubated with indicated concentrations of DSF–Cd and MG132 for 6 h, followed by a co-incubation for 2 h with the fluorogenic substrate Suc-LLVY-AMC (at 40 μmol/L) for the proteasomal chymotrypsin-like activity. After incubation, production of hydrolyzed AMC groups was measured. C, Morphological changes induced by DSF–Cd and MG132 at different concentrations. Columns, mean of independent triplicate experiments; bars, SD.