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. 2013 Aug 29;10:95. doi: 10.1186/1742-4690-10-95

Figure 3.

Figure 3

Four miRNAs reduce levels of full length and multiply spliced viral RNAs. Primary macaque macrophages were transfected with 100 nM of either miRNA mimics or antagonists, then infected with SIV (MOI 0.05) 24 hours after transfection. Cell lysates were collected at 24, 48 and 72 hours post-infection for miRNA experiments and 48 hours for miRNA antagonist experiments. Levels of full length (A, C) and spliced (B) RNA was measured by RT-qPCR. Values are expressed as fold change over SIV-only controls. Results are reported as percent of SIV-only control using the ΔΔCq method.