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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Apr 28;270(2):87–96. doi: 10.1016/j.taap.2013.04.014

Fig. 2.

Fig. 2

LAR dose-dependently down-regulates HDAC1 and HDAC5, but upregulates HDAC6 expression in RA synovial fibroblasts. (A) RA synovial fibroblasts (2 × 105/well) were incubated with LAR (0.5–5 μM) or SAHA (5 μM) in serum-free RPMI for 24 h to determine the effect on Class I and II HDACs by Western blotting method. (B) Densitometric analysis of the expression of different HDACs was quantified by determining the intensity of the bands using Image-J software (NIH). These values were normalized with the respective β-actin values and represented as the mean ± SEM of HDAC expression relative to untreated (NS) levels. The results represent values obtained from 5 independent donors’ cells under similar conditions. *p<0.05 vs NS group; **p<0.01 vs NS group.