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Chromatogram of the pass-through fraction and the eluted fraction from the anti-albumin and protein A and protein G′ columns when 20 μL of a 3-fold diluted serum was injected into the tandem affinity columns (the depletion and the lectin columns). The binding mobile phase consisted of 20 mM Tris, pH 7.4, containing 0.3 M NaCl and the eluting mobile phase for the depletion columns was made up of 50 mM NaH2PO4, pH 2.2. Flow rate, 0.8 mL/min.
