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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Proteomics. 2013 Apr 24;13(0):1701–1713. doi: 10.1002/pmic.201200524

Figure 3.

Figure 3

Chromatograms of (A) RPC fractionation of the LTA captured proteins and (B) RPC fractionation of the AAL captured proteins with depletion (D), and without depletion (ND) of albumin and Igs. The RPC fractionation was carried out by a linear gradient elution at increasing ACN concentration in the mobile phase by going from 0 % to 75 % of mobile phase B in 12 min. Mobile phase A consisted of H2O/ACN (95:5 v/v) containing 0.1% TFA and mobile phase B consisted of ACN/H2O (95:5 v/v) containing 0.1% TFA. Flow rate, 1 mL/min; UV detection, 214 nm.