Validation and confirmation of miRNAs expression by real-time quantitative PCR. qPCR demonstrated that rapamycin led to inverse expression patterns of miRNAs in Tg26 mice. Of these, miR-466i, miR-467f and miR-669a were up regulated and miR-497, miR-140, miR-145, miR-331, miR-16, miR-30a, miR-22, miR-30c, miR-378, miR-99a, miR-100a, miR-199a, miR-200a, miR-141, miR-200b, miR-200c and miR-429 were down regulated at 8 wks in HIVAN mice. U6 snRNA was used as an internal control. The relative expression level of specific miRNA was calculated using modified 2−ΔΔCt method.