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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Exp Cell Res. 2013 Apr 21;319(13):2073–2080. doi: 10.1016/j.yexcr.2013.04.011

Fig. 3.

Fig. 3

Validation and confirmation of miRNAs expression by real-time quantitative PCR. qPCR demonstrated that rapamycin led to inverse expression patterns of miRNAs in Tg26 mice. Of these, miR-466i, miR-467f and miR-669a were up regulated and miR-497, miR-140, miR-145, miR-331, miR-16, miR-30a, miR-22, miR-30c, miR-378, miR-99a, miR-100a, miR-199a, miR-200a, miR-141, miR-200b, miR-200c and miR-429 were down regulated at 8 wks in HIVAN mice. U6 snRNA was used as an internal control. The relative expression level of specific miRNA was calculated using modified 2−ΔΔCt method.