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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Cell Oncol (Dordr). 2013 Apr 26;36(3):247–257. doi: 10.1007/s13402-013-0132-x

Fig. 5.

Fig. 5

Apoptosis induction and beta-spectrin expression in Huh7 and 1MEA cells. a. Expression of survivin, LC3-I, LC3-II, cleaved caspase 7, cleaved caspase 9 and cleaved PARP in Huh7 cells treated with or without AIP-1 and AIP-2. b Expression of survivin, LC3-I, LC3-II, cleaved caspase 3, cleaved caspase 7 and cleaved PARP in 1MEA cells treated with or without AIP-1 and AIP-2. c Expression of beta-spectrin in Huh7 cells treated with or without AIP-1, AIP-2 and sorafenib. d After overnight culture, SNJ-1945, BT-1103 and EDTA were added to cells at different doses for 30 min, after which 10 µM AIP-1 was added. Cell lysates were resolved by SDS-PAGE and immunoblotted with a β II spectrin specific antibody. β-actin was used as a loading control. Representative Western blots are shown of β II spectrin breakdown products corresponding to 110 kD and 85 kD bands in 1MEA cells (upper panel) and Huh7 cells (lower panel)