Table 2.
Bacterial identification and screening for polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), and amidinotransferase (AMT) genes related to tetrodotoxin (TTX)-biosynthesis in bacterial isolates from Pleurobranchaea maculata.
| Strain | Closest 16S rRNA gene BLAST match a | Identity | PCR screening | TTX | ||
|---|---|---|---|---|---|---|
| (%) | PKS | NRPS | AMT | Detection b | ||
| PM DT05 | Photobacterium kishitanii strain S-27 (JF412253.1) | 99 | - | - | - | - |
| PM DT08 | Shewanella schlegeliana (AB081761.1) | 99 | - | - | - | - |
| PM DT10 | Photobacterium sp. DFC2.17 isolate DFC2.17 (FR873783.1) | 99 | - | - | - | - |
| PM DT11 | Photobacterium kishitanii strain S-27 (JF412253.1) | 99 | - | - | - | NT |
| PM DT12 | Shewanella schlegeliana (AB081761.1) | 99 | + | - | - | - |
| PM EG02 | Vibrio sp. YDO5 (GU586127.1) | 98 | - | - | - | - |
| PM EG04 | Staphylococcus warneri strain Na58 (HQ831387.1) | 99 | - | - | - | - |
| PM EG05 | Staphylococcus warneri strain Na59 (HQ831388.1) | 99 | - | - | - | - |
| PM EG08 | Pseudoalteromonas sp. 114Z-11 (GU584139.1) | 99 | - | - | - | - |
| PM EG09 | Pseudoalteromonas sp. BSs20138 (EU365489.1) | 99 | - | - | - | - |
| PM EG11 | Alteromonas sp. N98(2010) (HQ188650.1) | 99 | - | + | - | - |
| PM EG13 | Pseudoalteromonas sp. LJ1 (FJ665500.1) | 99 | - | - | - | - |
| PM EG14 | Halomonas sp. Pper-Hx-1972 (EU123940.1) | 99 | + | + | - | - |
| PM EG15 | Vibrio sp. S4639 (FJ457601.1) | 99 | - | - | - | - |
| PM EG17 | Pseudoalteromonas sp. 114Z-11 (GU584139.1) | 98 | - | - | - | - |
| PM EG18 | Pseudoalteromonas porphyrae strain HK1 (FJ205736.1) | 99 | + | + | - | - |
| PM GO01 | Shewanella schlegeliana (AB081761.1) | 99 | + | - | - | - |
| PM GO04 | Vibrio splendidus isolate PB1-10rrnM (EU091337.1) | 99 | - | - | - | - |
| PM GO05 | Photobacterium kishitanii strain calba.5.9 (AY642170.1) | 99 | - | - | - | - |
| PM GO06 | Shewanella schlegeliana (AB081761.1) | 99 | + | - | - | - |
| PM GO08 | Photobacterium kishitanii strain calba.5.9 (AY642170.1) | 99 | - | - | - | - |
| PM GO09 | Vibrio fischeri strain SI1E (AY292949.1) | 99 | - | - | - | NT |
| PM GO12 | Photobacterium kishitanii strain calba.5.9 (AY642170.1) | 99 | - | - | - | NT |
| PM GO13 | Vibrio fischeri strain SI1Ecomplete (AY292949.1) | 99 | - | - | - | NT |
| PM RT05 | Pseudoalteromonas sp. LJ1 (FJ665500.1) | 99 | - | + | - | - |
| PM RT07 | Alteromonas sp. N98(2010) (HQ188650.1) | 99 | - | + | - | - |
| PM RT10 | Vibrio parahaemolyticus strain NMGB2 (JN561593.1) | 99 | - | - | - | NT |
a NCBI Genbank accession numbers are indicated in brackets; b NT indicates that the sample was not tested for its ability to produce TTX. The detection limit of TTX using the LC-MS method was 0.1 ng/mL.