Table 4.
Screening of Nassarius semiplicatus bacterial isolates for putative TTX-biosynthesis genes and a comparison of ELISA and LC-MS methods for the detection of TTX.
| Strain a | PCR screening | TTX detection (ELISA) | TTX detection | ||
|---|---|---|---|---|---|
| PKS | NRPS | AMT | (ng/g) b | (LC-MS) | |
| Vibrio sp. 34HU9 (EU268265) | + | + | − | 169 | BDL c |
| Marinomonas sp. 38JIA1 (EU268259) | − | + | − | 98 | BDL |
| Tenacibaculum sp. 30ORI8 (EU268261) | − | + | − | 54 | BDL |
a Strains are as described in Wang, X., et al., 2008 [8]; b ELISA results shown are those previously reported by Wang, X., et al. 2008 [8]. The detection limit of TTX using the ELISA method was 5 ng/mL (equivalent to 15 ng/g of TTX in tissue); c BDL indicates that the concentration of TTX was below the detectable limit of the instrumentation. The detection limit of TTX using the LC-MS method was 0.1 ng/mL (equivalent to 0.3 ng/g of TTX in tissue).