Table 5.
Primers used for identification and screening of bacterial isolates.
| Primer | Sequence | Tma | ATb | Reference |
|---|---|---|---|---|
| 27fl | AGAGTTTGATCCTGGCTCAG | 61 | 55 | [41] |
| 1494rc | TACGGCTACCTTGTTACGAC | 59 | 55 | [41] |
| DKF | GTGCCGGTNCC(A/G)TGNG(T/C)(T/C)TC | 67 | 55 | [42] |
| DKR | GCGATGGA(T/C)CCNCA(A/G)CA(A/G)(C/A)G | 65 | 55 | [42] |
| MTF2 | GCNGG(C/T)GG(C/T)GCNTA(C/T)GTNCC | 64 | 52 | [43] |
| MTR2 | CCNCG(A/G/T)AT(T/C)TTNAC(T/C)TG | 47 | 52 | [43] |
| ATfwd1 | GT(A/C/G)TG(T/C)CC(A/T)(A/C)G(G/C)GA(T/C)GT(A/C/G)ATG | 57 | 55 | [44] |
| ATrev1 | AT(A/G)TCCCA(A/T)(A/G)T(C/G/T)C(A/G)CA(A/G)TG | 62 | 55 | [44] |
a Tm is the theoretical melting temperature of the PCR primers, given in °C; b AT is the annealing temperature used in PCRs containing these primers, given in °C.