Figure 2.

Decrease of the substrate aeroplysinin-1 (in %) relative to a control without enzyme for fractions derived from differential centrifugation at 800 g and 100,000 g. The supernatant S1 and pellet P1 resulted from a centrifugation step at 800 g, while the supernatant S2 and pellet P2 were obtained from a centrifugation step at 100,000 g. Activity (in %) is defined as the decrease of substrate relative to a control lacking the enzyme fraction. The activity is calculated for 10 µg of protein. (One representative experiment shown).