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Purification of A. cavernicola nitrile hydratases from 10 g of lyophilized sponge tissue.
| Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|
| Cell-free extract | 114.1 | 36.1 | 0.31 | 100 |
| Ammonium sulphate fractionation | 30.9 | 10.8 | 0.35 | 29.9 |
| HiTrap Q Sepharose XL | 2.4 | 1.08 | 0.45 | 2.9 |
| Superdex 200 10/300 | 0.05 | 0.81 | 16.2 | 1.3 |
