Table 2.

Peptide masses obtained from de-novo sequencing by tandem mass spectrometry. The table shows the molecular mass ([M + H]⁺), the charge (z) and the mass-charge ratio (m/z) of the peptide fragments obtained from tandem mass spectrometry (MS/MS) along with the sequence of the fragments. The amino acid sequences are given in single letter code. Sequences in parenthesis denote amino acids of which the order could not be determined experimentally [e.g., (FN) is either FN or NF]. Furthermore it is not possible to differentiate L and I by mass spectrometry due to the identical mass, therefore the peptide fragments contain only L. Similarly, it is very difficult to distinguish Q and K by mass spectrometry, therefore within a peptide Q is reported as K would have been a cleavage site for trypsin and thus can only be found at the end of the peptide (assuming complete cleavage).

Molecular weight ([M + H]+)	Charge (z)	Mass-charge ratio (m/z)	Sequence
914.46	++	457.73	LSSEFGFK
960.56	++	480.78	FVTPLDLR
1187.64	++	594.37	WDETVVALVR
1937.92	+++	646.64	(FN)FDLTHQQQLDYLR
2269.15	+++	757.05	DLPASANDLPYFLLHAQLDR