TABLE 1.
Tcrb-VDJ sequences from single cells demonstrate clonality of donor populations
| Frequency | Tcrb-V | Tcrb-D | Tcrb-J | CDR3β | |
|---|---|---|---|---|---|
| Random CD4+ | 1 of 10 | 3 | 1 | 1–1 | CASSLDTEVFF |
| 1 of 10 | 5 | 2 | 2–3 | CASRGLGEAETLYF | |
| 1 of 10 | 5 | 2 | 2–5 | CASSQEDWGGRDTQYF | |
| 1 of 10 | 13–1 | 2 | 2–5 | CASSGDWGAQDTQYF | |
| 1 of 10 | 13–1 | 2 | 2–7 | CASSDVGGYEQYF | |
| 1 of 10 | 13–2 | 2 | 2–5 | CASGGLGGRTQYF | |
| 1 of 10 | 13–2 | 1 | 1–2 | CASGDQGQGAGSDYTF | |
| 1 of 10 | 15 | 1 | 2–5 | CASRGHLQDTQYF | |
| 1 of 10 | 15 | 1 | 1–1 | CASSLQGANTEVFF | |
| 1 of 10 | 16 | 1 | 1–3 | CASSSRISGNTLYF | |
| Polyclonal LLOp:I-Ab-specific | 9 of 22 | 14 | 1 | 1–6 | CASSRQGSYEQYF |
| 4 of 22 | 4 | 2 | 2–7 | CASSRDWGGYEQYF | |
| 3 of 22 | 14 | 1 | 1–1 | CASSFQGAEVFF | |
| 2 of 22 | 15 | 1 | 1–1 | CASRPGQGGRSLF | |
| 1 of 22 | 19 | 2 | 2–7 | CASSIALGTGYEQYF | |
| 1 of 22 | 5 | 1 | 2–5 | CASSPQGDTQYF | |
| 1 of 22 | 5 | 2 | 2–7 | CASSHSDWGAYEQYF | |
| 1 of 22 | 1 | 1 | 2–2 | CTCSAGQANTGQLYF | |
| Donor Population 1 | 13 of 13 | 19 | 2 | 2–5 | CASSPQGSGNTLYF |
| Donor Population 2 | 7 of 7 | 14 | 2 | 2–7 | CASSSIYEQYF |
| Donor Population 3 | 18 of 18 | 13-3 | 2 | 2–4 | CASTALGNQNTLYF |
| Donor Population 4 | 7 of 7 | 13-1 | 1 | 1–6 | CASSDLQYNSPLYF |
The sequences shown were from single randomly selected CD4+ T cells (Random CD4+), single LLOp:I-Ab-tetramer binding CD4+ T cells from day 7 Lm infected mice (Polyclonal LLOp:I-Ab-specific), or from single cells of donor origin in day 7 Lm infected mice that received limiting numbers of donor cells (Donor Population 1–4).