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. 2013 Sep 9;4:269. doi: 10.3389/fimmu.2013.00269

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Copyright © 2013 Tian, Matsuo, Fukunaga, Ono, Nishigori and Yodoi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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After stimulation with croton oil, rhTRX suppressed the release of TNF-α, IL-6, and MCP-1 by PAM 212 cells into the medium. PAM 212 cells were left untreated (no croton oil) or stimulated with croton oil in the presence of BSA or rhTRX (10 μg/ml). The concentrations of TNF-α, IL-6, and MCP-1 in the culture supernatant were measured by CBA assay 24 h after croton oil treatment. The concentrations of TNF-α, IL-6, and MCP-1 were significantly lower than those shown in the BSA application group (*P < 0.05). Values are shown as the mean ± SD of the samples (Student t-test).