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Western blot analysis of iNOs and ZAP-70 in MT4 cells. Ingenol treatment during HIV infection resulted in recovery of iNOS and ZAP-70. MT-4 cells were infected with HIV-1IIIB at multiplicity of infection (MOI; 0.004 [50 TCID50]) after 5 μM ingenol treatment then protein expression in cell lysate was analyzed by western blot 24 hours after HIV infection. iNOS, inducible nitric oxide synthase; ZAP-70, zeta chain-associated protein kinase 70. β-actin was used as loading control.
