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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Nat Med. 2012 Jun;18(6):943–949. doi: 10.1038/nm.2756

Figure 1.

Figure 1

NIK is overactivated in the livers of mice with obesity. (a) NIK in liver extracts was immunoprecipitated with antibody to NIK and subjected to in vitro kinase assays. The blots were subsequently immunoblotted (IB) with antibody to GST and NIK. (b) Males (7 weeks) were fed a normal chow diet or an HFD for 6 weeks. NIK in liver extracts was immunopurified with antibody to NIK and subjected to in vitro kinase assays. (c) Liver extracts were immunoblotted with antibody to NF-κB2 or Tubulin. (d) Primary hepatocytes were infected with Flag-tagged NIK adenoviruses. Sixteen hours after infection, cells were treated for 2 h with a vehicle (Con) or TNF-α (10 ng ml−1), H2O2 (100 nM) or PA (100 µM). NIK was immunoprecipitated with antibody to Flag and subjected to in vitro kinase assays. The blots were immunoblotted with antibody to Flag and GST.