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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4750–4759. doi: 10.4049/jimmunol.1000769

FIGURE 3.

FIGURE 3

(A) Concentration-dependent inhibitory effects of TGF-β1/Fc on IL-4-dependent HT-2 cell proliferation. HT-2 cells were stimulated with rIL-4 and cultured in the presence of the indicated concentrations of rTGF-β1, TGF-β1/Fc or IgG4 for 24 h. [3H] thymidine incorporation was measured. Data are expressed as the mean percentage of inhibition from three independent experiments. (B) Western blot analysis for the expression of pSmad2 and Smad2 in HT-2 cells treated with rTGF-β1, TGF-β1/Fc or IgG4 for 24 h. The same lysates were also used to evaluate the expression of β-actin as loading control. One of two similar experiments is shown.