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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Nat Immunol. 2010 Dec 26;12(2):129–136. doi: 10.1038/ni.1978

Figure 3.

Figure 3

Ldb1−/− fetal hematopoietic progenitor (LSK) populations do not contain LTR-HSCs. (a) Fetal liver LSK cells (left) in embryos at E12.5: Tie2-Cre Ldb1fl/Δ, 1.87 × 104 ± 0.61 × 104; control (Ldb1fl/Δ, Ldb1+/fl and Tie2-Cre Ldb1+/fl littermates), 1.68 × 104 ± 0.57 × 104 (mean ± s.d.). P = 0.52 (Student’s t-test). Middle, flow cytometry of Linlo–neg fetal liver cells from Tie2-Cre Ldb1+/fl and Tie2-Cre Ldb1fl/Δ littermates at E12.5, stained for c-Kit and Sca-1 (left), followed by analysis of Flt3 expression (right) in the gate outlined at left (LSK cells; arrows). Numbers below outlined areas indicate percent LSK cells (left plots) or percent HSCs (left area; Flt3−) and MPPs (right area; Flt3+) among LSK cells (right plots). Far right, flow cytometry of fetal liver LSK cells at E12.5; numbers adjacent to outlined areas indicate percent CD48−CD150+ LSK cells. Data are from one representative of two experiments. (b) Flow cytometry of donor-derived (CD45.2+) cells in irradiated Rag2−/− (CD45.1) hosts 16 weeks after adoptive transfer of total fetal liver cells from Tie2-Cre Ldb1+/fl or Tie2-Cre Ldb1fl/Δ mice at E12. Numbers adjacent to outlined areas indicate percent in each gate. Data are from one representative of two experiments.