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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Nat Immunol. 2010 Dec 26;12(2):129–136. doi: 10.1038/ni.1978

Figure 4.

Figure 4

Ldb1 is continuously required for the maintenance of adult HSCs. (a) LSK profile (left) of bone marrow cells from mice injected with poly(I:C) on days 1, 3 and 5, assessed on day 12 by staining of gated Linlo–neg bone marrow cells for c-Kit and Sca-1. Control, Ldb1fl/fl, Ldb1+/fl and Mx1-Cre Ldb1+/fl mice. Numbers above outlined areas indicate percent LSK cells; numbers above plots indicate mice with a phenotype similar to that shown in the plot/total mice. Right, total bone marrow and LSK cells from control mice (n = 14) and Mx1-Cre Ldb1fl/fl mice (n = 11). *P < 0.05 (Student’s t-test). Data are from one representative of five experiments (error bars, s.d.). (b) Flt3 expression (left) by LSK cells from adult Ldb1fl/fl or Mx1-Cre Ldb1fl/fl littermates injected with poly(I:C) on days 1, 3 and 5, assessed on day 6. Numbers adjacent to outlined areas indicate percent Flt3− LSK cells (HSC) or Flt3+ LSK cells (MPP). Right, total bone marrow and LSK cells from control mice (n = 3) and Mx1-Cre Ldb1fl/fl mice (n = 3). *P < 0.05 and **P < 0.01 (Student’s t-test). Data are representative of two experiments (error bars, s.d.). (c) Flow cytometry (left) of bone marrow cells and thymocytes from irradiated Rag2−/− (CD45.1) recipient mice 6 months after injection of 50:50 mixtures of bone marrow cells from Mx1-Cre Ldb1fl/fl (CD45.2) mice or littermate control (CD45.2) mice and B6 (CD45.1) mice that had been injected with poly(I:C) as described in a. Numbers adjacent to outlined areas indicate percent cells in each gate. Right, summary of data at left. Data are from one representative of three independent experiments (error bars, s.d.). (d) CD45.2+ cells among bone marrow cells from premade bone marrow chimeras generated with 50:50 mixtures of bone marrow cells from Mx1-Cre Ldb1fl/fl (CD45.2) mice or control Mx1-Cre Ldb1+/fl (CD45.2) mice and bone marrow cells from B6 (CD45.1) mice after injection of poly(I:C) (three times, every other day). CMP, common myeloid progenitor, GMP, granulocyte macrophage progenitor. Data are from one representative of two experiments.