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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Nat Immunol. 2010 Dec 26;12(2):129–136. doi: 10.1038/ni.1978

Figure 5.

Figure 5

Ldb1 regulates the expression of many transcription factors required for HSC maintenance. (a) Expression of transcripts encoding Ldb1 and subunits of the Ldb1 complex in hematopoietic progenitors. T, total adult bone marrow; LK, sorted Linlo–neg c-Kit+ bone marrow; LSK, sorted LSK bone marrow. Data are from one representative of two independent experiments. (b) Real-time RT-PCR analysis of various transcripts (horizontal axis) in bone marrow cells obtained from adult Mx1-Cre Ldb1fl/fl mice or control Mx1-Cre Ldb1+/+ mice 2 d after the first of two injections of poly(I:C) (day 3), then enriched for hematopoietic progenitors (lineage depletion). Results in a and b are presented relative to expression of mRNA for the endogenous reference gene Actb. Below, genes with (+) or without (−) Ldb1-, Scl- and/or GATA-2-binding sites in the gene body or within 5 kb of the transcription start site, assessed by ChIP-Seq. *P < 0.05 and **P < 0.01 (Student’s t-test). Data are representative of three independent experiments (error bars, s.d.).