Abnormal expression of proteins involved in brain development and synaptic plasticity in the forebrain of newborn homozygous NEX-Pten mice. Representative Western blots show upregulation of Dab1 (a), MAP2 and NeuN (c), NR2A, NR2B and GluR1 (e) in homozygous (Hom) compared to heterozygous (Het) and wild type (WT) mice. Quantitative analysis of data obtained from 5–6 mice per genotype confirms that Dab1 (b), MAP2 (d), NR2A and NR2B levels (f) normalized to actin are significantly higher in homozygous than in wild type samples (p<0.05).