Table 2.

Relative efficiency and cost-savings of Minipool+algorithm strategy for pooled viral load testing of Archived samples.

Time Post- HAART	No. samples	Viral Failure (95% CI)	No. pools	No. individual assays performed	Total no. assays performed	No. assays saved	Relative Efficiency (%) (95% CI)	Cost savings (per 100 assays saved)
Overall	250	13.6 (9.6–18.5)	50	141	191	59	23.6 (18.5–29.4)	$1,180
6 month	49*	12.2	10	22	32	17	35 (22–50)	$1,735
1–2 years	50	12.0	10	25	35	15	30 (18–45)	$1,500
2–3 years	50	16.0	10	40	50	0	0 (0–7)	$0
3–4 years	50	14.0	10	29	39	11	22 (12–36)	$1,100
4–5 years	50	14.0	10	24	34	15	30 (18–45)	$1,500

A total of 250 archived plasma samples were obtained for ART monitoring and pooled in groups of 5 samples according to time post-ART for detection of virologic failure.

^*After compilation and testing of pools, one sample was found to be out of the desired range and is excluded from the 6 month time interval.