Skip to main content
. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Mol Biochem Parasitol. 2011 Sep 22;181(1):7–16. doi: 10.1016/j.molbiopara.2011.09.006

Figure 2. Immunofluorescent analysis of mutant 13-20C2 reveals severe defects in chromosome segregation and nuclear division.

Figure 2

Immunofluorescent microscopy of parental strain RHΔhxgprt (a, b, c) and mutant 13-20C2 was performed 16 h post-infection at 34°C (d, e, f) and 40°C (g, h, I, k, l, m) to determine the primary temperature defect. Representative images showing the co-staining with anti-IMC1 (green) antibodies and DAPI (blue) revealed the primary retention of genomic DNA in the mother residual mass (dashed circled) occurred in parasites grown at the non-permissive temperature (40°C). The loss of chromosome material was variable with some parasites retaining significant DNA (middle panels), while other newly formed parasites lacked detectable DAPI staining (lower panels). Magnification bar (5 μm) is indicated in the lower right hand Nomarski image.