Table 2.
Summary of the properties of GP64 truncations
C-terminally truncations detected by:
|
N-terminally truncations detected by
|
||||||
---|---|---|---|---|---|---|---|
WB anti-His | IP AcV1 | Refolding AcV1 | WB anti-His | IP AcV1 | Refolding AcV1 | ||
CTr1(21-482) | + | + | + | NTr1(21-512) | + | + | + |
CTr2(21-435) | + | + | + | NTr2(125-512) | + | + | + |
CTr3(21-294) | + | + | + | NTr3(186-512) | + | + | + |
CTr4(21-273) | + | − | − | NTr4(264-512) | + | − | + |
CTr5(21-217) | + | − | − | NTr5(271-512) | + | − | + |
CTr6(21-157) | + | − | − | NTr6(377-512) | + | − | − |
CTr7(21-131) | + | − | − | NTr7(404-512) | + | − | − |
CTr8(21-98) | + | − | − | NTr8(459-512) | + | − | − |
CTr9(21-58) | + | − | − | NTr9(481-512) | + | − | − |
WB, Western Blot. IP, Immunoprecipitation. Refolding, Refolding Assay.
The numbers in parentheses indicate the positions of amino acids covering the peptides.