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. Author manuscript; available in PMC: 2013 Sep 9.
Published in final edited form as: Soft Matter. 2012 Aug 14;8(30):7909–7918. doi: 10.1039/C2SM25779C

Figure 5.

Figure 5

Competitive binding of CRP to oxLDL using LPP mimics. Air oxidized LDLs (5.7 μg/mL) were immobilized on ELISA plates overnight at 4°C. Equal amounts of CRP (3.6 μg/mL) were pre-incubated for 30 min with LPP mimics: 21 nm, 26 nm, 28 nm, 41 nm, 64 nm, and PC liposome, or PCh-BSA, pCRP and mCRP controls. Then the CRP-LPP mimics were added to the oxLDL-coated plates. The binding of CRP to oxLDL was detected using biotinylated polyclonal anti-CRP antibody and the measured absorbance was normalized to pCRP. Inset: Competitive immunoassay using PCh-BSA. Data represents mean absorbance ±SD, n = 5. * p<0.05; ***p<0.001 compared to pCRP.