Figure 6.
Representative topographic AFM images of LDL immobilized on ELISA well. AFM images of (A) high binding ELISA well, (B) antibody-captured LDLs and (C) direct immobilized LDLs on ELISA well. LDLs (5.7 μg/mL) were captured using a anti-Apo B polyclonal antibody or directly immobilized onto high binding ELISA well and incubated overnight at 4°C. After rinsing and drying, images were acquired in air using a tapping mode AFM. Insets show a cartoon illustrating the probable structures of the respective surfaces. Image scan size = 5 × 5μm.